neuregulin β  (R&D Systems)

Journal: Journal of Biomedicine and Biotechnology

Article Title: Expressions of Neuregulin 1 β and ErbB4 in Prefrontal Cortex and Hippocampus of a Rat Schizophrenia Model Induced by Chronic MK-801 Administration

doi: 10.1155/2010/859516

Figure Lengend Snippet: Expression of NRG1 β and ErbB4 proteins in the rat prefrontal cortex. (a) Representative Western blots of NRG1 β protein isoforms. (b) Quantitative analysis of NRG1 β immunoblots. (c) Representative Western blots of ErbB4 proteins isoforms. (d) Quantitative analysis of ErbB4 immunoblots. The data were normalized by taking the value of vehicle group as 100% and expressed as means ± S.E.M. ( n = 5). * P < .05. (e) Representative section of ErbB4 immunohistochemistry in the PrL region of prefrontal cortex from vehicle group. (f) Representative section of ErbB4 immunohistochemistry in the PrL region of prefrontal cortex from MK-801-treated group. (g) Representative section of ErbB4 immunohistochemistry in the Cg1 and M2 region of prefrontal cortex from vehicle group. (h) Representative section of ErbB4 immunohistochemistry in the Cg1 and M2 region of prefrontal cortex from MK-801-treated group. Arrows indicate ErbB4-expressing neurons in the prefrontal cortex. Scale bar for (e)–(h): 50 μ m.

Article Snippet: The signal of the 65 kDa NRG1 β isoform was reduced and labeling of the other NRG1 β isoforms and ErbB4 isoforms was eliminated by preabsorption with corresponding antigen peptides (Santa Cruz Biotechnology Inc.).

Techniques: Expressing, Western Blot, Immunohistochemistry

Journal: Journal of Biomedicine and Biotechnology

Article Title: Expressions of Neuregulin 1 β and ErbB4 in Prefrontal Cortex and Hippocampus of a Rat Schizophrenia Model Induced by Chronic MK-801 Administration

doi: 10.1155/2010/859516

Figure Lengend Snippet: Immunoreactivity of NRG1 β and ErbB4 in the rat prefrontal cortex and hippocampal subregions.

Article Snippet: The signal of the 65 kDa NRG1 β isoform was reduced and labeling of the other NRG1 β isoforms and ErbB4 isoforms was eliminated by preabsorption with corresponding antigen peptides (Santa Cruz Biotechnology Inc.).

Techniques:

Journal: Journal of Biomedicine and Biotechnology

Article Title: Expressions of Neuregulin 1 β and ErbB4 in Prefrontal Cortex and Hippocampus of a Rat Schizophrenia Model Induced by Chronic MK-801 Administration

doi: 10.1155/2010/859516

Figure Lengend Snippet: Expression of NRG1 β and ErbB4 proteins in the rat hippocampus. (a) Representative Western blots of NRG1 β protein isoforms. (b) Quantitative analysis of NRG1 β immunoblots. (c) Representative Western blots of ErbB4 protein isoforms. (d) Quantitative analysis of ErbB4 immunoblots. The data were normalized by taking the value of vehicle group as 100% and expressed as means ± S.E.M. ( n = 5). * P < .05. (e) Representative section of NRG1 β immunohistochemistry in the hippocampal CA1 region from vehicle group. (f) Representative section of NRG1 β immunohistochemistry in the hippocampal CA1 region from MK-801-treated group. (g) Representative section of NRG1 β immunohistochemistry in the hippocampal CA3 region from vehicle group. (h) Representative section of NRG1 β immunohistochemistry in the hippocampal CA3 region from MK-801-treated group. (i) Representative section of NRG1 β immunohistochemistry in the hippocampal DG region from vehicle group. (j) Representative section of NRG1 β immunohistochemistry in the hippocampal DG region from MK-801-treated group. Arrows indicate NRG1-expressing neurons in the hippocampus. Scale bar for (e)–(j): 50 μ m.

Article Snippet: The signal of the 65 kDa NRG1 β isoform was reduced and labeling of the other NRG1 β isoforms and ErbB4 isoforms was eliminated by preabsorption with corresponding antigen peptides (Santa Cruz Biotechnology Inc.).

Techniques: Expressing, Western Blot, Immunohistochemistry

Journal: Brain Pathology

Article Title: Accumulation of misfolded SOD1 outlines distinct patterns of motor neuron pathology and death during disease progression in a SOD1 G93A mouse model of amyotrophic lateral sclerosis

doi: 10.1111/bpa.13078

Figure Lengend Snippet: Antibodies used for immunocytochemistry

Article Snippet: NRG1 1α /β 1/2 , Rabbit polyclonal , Santa Cruz (sc‐348) , 1:300.

Techniques: Ubiquitin Proteomics

Journal: Brain Pathology

Article Title: Accumulation of misfolded SOD1 outlines distinct patterns of motor neuron pathology and death during disease progression in a SOD1 G93A mouse model of amyotrophic lateral sclerosis

doi: 10.1111/bpa.13078

Figure Lengend Snippet: C‐type synaptic boutons and mfSOD1 MN phenotypes in SOD1 G93A mice. (A) C‐type synapses in MN somata (delimited by a line drawn on a not shown Nissl image) were analyzed after labeling their postsynaptic and presynaptic compartments to visualize NRG1 (green) and VAChT (red), respectively, in WT and P60 SOD1 G93A , as indicated. C‐Bouton imaging was merged with mfSOD1 immunostaining (gray) to identify the three MN phenotypes. (B) Changes in the size of pre‐ and postsynaptic components of C‐boutons evaluated at distinct disease ages in relation to MN mfSOD1 phenotypes vs control. (C) Effects of treatments with the M2 muscarinic cholinergic receptor agonist (oxotremorine, ox) or antagonist (methoctramine, meth) of P60 SOD1 G93A mice on the expression of mfSOD1 and C‐Bouton size 15 days after treatment. Sample sizes were as follows: WT, n = 193; SOD1 G93A , n = 219; ox, n = 167; meth, n = 202; n = synapse number s from 3 animals. One‐way ANOVA: p < 0.0001, ox vs. control; p < 0.01, meth vs. control data for presynaptic C‐Bouton density were as follows: WT, n = 37; SOD1 G93A , n = 59; ox, n = 46; meth, n = 24 VAChT‐positive puncta/100 μm 2 ; n = number of MNs from 3 animals. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, one‐way ANOVA, Bonferroni's post hoc test. Scale bar: (A) = 20 μm.

Article Snippet: NRG1 1α /β 1/2 , Rabbit polyclonal , Santa Cruz (sc‐348) , 1:300.

Techniques: Labeling, Imaging, Immunostaining, Control, Expressing